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cy5 conjugated goat anti rabbit secondary antibody  (Jackson Immuno)


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    Jackson Immuno cy5 conjugated goat anti rabbit secondary antibody
    Cy5 Conjugated Goat Anti Rabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 709 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy5+conjugated+donkey+anti+goat/Cy+5+AffiniPure+Donkey+Anti-Rabbit+IgG/pm41899598-111-53-58
    Average 96 stars, based on 709 article reviews
    cy5 conjugated goat anti rabbit secondary antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctions
    Article Snippet: The following secondary antibodies were used: Alexa 488-conjugated donkey anti-rabbit (A-21206, 1:300, Invitrogen); Alexa 488-conjugated donkey anti-goat (A-11055, 1:300, Invitrogen); Alexa 488-conjugated donkey anti-rat (A-21208, 1:300, Invitrogen); Cy3-conjugated donkey anti-rabbit (711-165-152, 1:300, Jackson ImmunoResearch); Cy3-conjugated donkey anti-mouse (715-165-151, 1:300, Jackson ImmunoResearch); Cy3-conjugated donkey anti-goat (705-165-147, 1:300, Jackson ImmunoResearch); Cy5-conjugated donkey anti-goat (705-495-147, 1:300, Jackson ImmunoResearch) and Cy5-conjugated donkey anti-rat (712-175-150, 1:300, Jackson ImmunoResearch).

    Transfection:

    Article Title: Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctions.
    Article Snippet: Antibodies The following primary antibodies were used: rabbit anti-Prox1 (11002, 1:500, AngioBio); goat anti-Prox1 (AF2727, 1:200, R&D Systems); rat anti-PECAM1 (553370, 1:100, BD Pharmingen); goat anti-Lyve1 (AF2125, 1:200, R&D Systems); rat anti-VE-cadherin (clone: 11D4.1, 555289, 1:200, BD Pharmingen); rat anti-VE-cadherin (clone: BV13A, 1:200, ImClone Systems); rabbit anti-VE-cadherin (ab33168, 1:500, Abcam); goat anti-integrin α9 (AF3827, 1:100, R&D Systems); goat antiVEGFR3 (AF743, 1:100, R&D Systems); goat anti-Nrp2 (AF567, 1:500, R&D Systems); rabbit anti-ZO-1 (40-2200, 1:200, Invitrogen); rabbit antiKi67 (clone: SP6, MA5-14520, 1:200, Invitrogen); rabbit anti-active caspase 3 (clone: C92-605, 559565, 1:200, BD Pharmingen); mouse anti- D E V E LO P M E N T paxillin (clone: 349, 610051, 1:200, BD Transduction Laboratories); rat anti-integrin β1 (clone: 9EG7, 550531, 1:200, BD Biosciences); rabbit-anti p-paxillin (2541, 1:500, Cell Signaling Technology); goat anti-Rasip1 (ab21018, 1:500, Abcam); mouse anti-Rac1 (ARC03, 1:100, Cytoskeleton); Cdc42 (ACD03,1:100, Cytoskeleton); RhoA (ARH04, 1:100, Cytoskeleton); rabbit anti-Gapdh (clone: 6C5, sc-32233, 1:1000, Santa Cruz Biotechnology). .. The following secondary antibodies were used: Alexa 488-conjugated donkey anti-rabbit (A-21206, 1:300, Invitrogen); Alexa 488-conjugated donkey anti-goat (A-11055, 1:300, Invitrogen); Alexa 488- conjugated donkey anti-rat (A-21208, 1:300, Invitrogen); Cy3-conjugated donkey anti-rabbit (711-165-152, 1:300, Jackson ImmunoResearch); Cy3conjugated donkey anti-mouse (715-165-151, 1:300, Jackson ImmunoResearch); Cy3-conjugated donkey anti-goat (705-165-147, 1:300, Jackson ImmunoResearch); Cy5-conjugated donkey anti-goat (705-495-147, 1:300, Jackson ImmunoResearch) and Cy5-conjugated donkey anti-rat (712-175-150, 1:300, Jackson ImmunoResearch). siRNA transfection Human dermal LECs were purchased from PromoCell and cultured in endothelial basal medium (EBM) complemented with supplement mix (C-39225, PromoCell) according to the supplier’s instructions. .. Human RASIP1 siRNA or CDC42 siRNAs were obtained from GE Dharmacon and were transfected into cultured human dermal LECs using Lipofectamine 2000 (Thermo Fisher Scientific) as has previously been described (Srinivasan et al., 2014).

    Cell Culture:

    Article Title: Rasip1 controls lymphatic vessel lumen maintenance by regulating endothelial cell junctions.
    Article Snippet: Antibodies The following primary antibodies were used: rabbit anti-Prox1 (11002, 1:500, AngioBio); goat anti-Prox1 (AF2727, 1:200, R&D Systems); rat anti-PECAM1 (553370, 1:100, BD Pharmingen); goat anti-Lyve1 (AF2125, 1:200, R&D Systems); rat anti-VE-cadherin (clone: 11D4.1, 555289, 1:200, BD Pharmingen); rat anti-VE-cadherin (clone: BV13A, 1:200, ImClone Systems); rabbit anti-VE-cadherin (ab33168, 1:500, Abcam); goat anti-integrin α9 (AF3827, 1:100, R&D Systems); goat antiVEGFR3 (AF743, 1:100, R&D Systems); goat anti-Nrp2 (AF567, 1:500, R&D Systems); rabbit anti-ZO-1 (40-2200, 1:200, Invitrogen); rabbit antiKi67 (clone: SP6, MA5-14520, 1:200, Invitrogen); rabbit anti-active caspase 3 (clone: C92-605, 559565, 1:200, BD Pharmingen); mouse anti- D E V E LO P M E N T paxillin (clone: 349, 610051, 1:200, BD Transduction Laboratories); rat anti-integrin β1 (clone: 9EG7, 550531, 1:200, BD Biosciences); rabbit-anti p-paxillin (2541, 1:500, Cell Signaling Technology); goat anti-Rasip1 (ab21018, 1:500, Abcam); mouse anti-Rac1 (ARC03, 1:100, Cytoskeleton); Cdc42 (ACD03,1:100, Cytoskeleton); RhoA (ARH04, 1:100, Cytoskeleton); rabbit anti-Gapdh (clone: 6C5, sc-32233, 1:1000, Santa Cruz Biotechnology). .. The following secondary antibodies were used: Alexa 488-conjugated donkey anti-rabbit (A-21206, 1:300, Invitrogen); Alexa 488-conjugated donkey anti-goat (A-11055, 1:300, Invitrogen); Alexa 488- conjugated donkey anti-rat (A-21208, 1:300, Invitrogen); Cy3-conjugated donkey anti-rabbit (711-165-152, 1:300, Jackson ImmunoResearch); Cy3conjugated donkey anti-mouse (715-165-151, 1:300, Jackson ImmunoResearch); Cy3-conjugated donkey anti-goat (705-165-147, 1:300, Jackson ImmunoResearch); Cy5-conjugated donkey anti-goat (705-495-147, 1:300, Jackson ImmunoResearch) and Cy5-conjugated donkey anti-rat (712-175-150, 1:300, Jackson ImmunoResearch). siRNA transfection Human dermal LECs were purchased from PromoCell and cultured in endothelial basal medium (EBM) complemented with supplement mix (C-39225, PromoCell) according to the supplier’s instructions. .. Human RASIP1 siRNA or CDC42 siRNAs were obtained from GE Dharmacon and were transfected into cultured human dermal LECs using Lipofectamine 2000 (Thermo Fisher Scientific) as has previously been described (Srinivasan et al., 2014).

    Incubation:

    Article Title: Microglia maintain structural integrity during fetal brain morphogenesis
    Article Snippet: Slices were first incubated for 1 h at room temperature (RT) in 0.2% Triton X-100, 0.2% Gelatin in PBS (blocking solution), and then incubated at 4°C overnight in the same blocking solution with the following primary antibodies: rat anti-CD68 (1/500; Bio-Rad Cat# MCA1957, RRID: AB_322219 ), rabbit anti-FN1 (1/200; Millipore Cat# AB2033, RRID: AB_2105702 ), goat anti-FOXP2 (1/500; Santa Cruz Biotechnology Cat# sc-21069, RRID: AB_2107124 ), chicken anti-GFP (1/1000; Aves Labs Cat# GFP-1020, RRID: AB_10000240 ), rabbit anti-IBA1 (1/500; FUJIFILM Wako Shibayagi Cat# 019-19741, RRID: AB_839504 ), rabbit anti-IBA1 (human) (1/500; Abcam Cat# ab178846, RRID: AB_2636859 ), chicken anti-IBA1 (1/500; Synaptic Systems Cat# 234009, RRID: AB_2891282 ), rat anti-Lgals3 (MAC2) (1/1000; CEDARLANE Cat# CL8942AP, RRID: AB_10060357 ), rat anti-L1 (1/100; Millipore Cat# MAB5272, RRID: AB_2133200 ), biotinylated rat anti-LYVE1 (1/200; Thermo Fisher Scientific Cat# 13-0443-82, RRID: AB_1724157 ), rat anti-Myelin Basic Protein (MBP) (1/300; Millipore Cat# MAB386, RRID: AB_94975 ), rat anti-mDectin-1 (CLEC7A) (1/30; InvivoGen Cat# mabg-mdect, RRID: AB_2753143 ), mouse anti-neurofilament marker SMI-312 (1/300; BioLegend Cat# 837904, RRID: AB_2566782 ), goat anti-mouse Osteoactivin (GPNMB) (1/200; R and D Systems Cat# AF2330, RRID: AB_2112934 ), rabbit anti-P2Y12 receptor (1/500; AnaSpec; EGT Group Cat# 55043A, RRID: AB_2298886 ) and goat anti-SPP1 (1/400; R and D Systems Cat# AF808, RRID: AB_2194992 ). .. Sections were rinsed in PBS-0.1% TritonX-100 and incubated at 4°C from 2 h to overnight with the following secondary antibodies (1/400 in PBS, Jackson ImmunoResearch Labs): Alexa 10 Fluor® 488-conjugated donkey anti-chicken (Cat# 703-545-155, RRID: AB_2340375 ), Alexa 10 Fluor® 488-conjugated donkey anti-goat (Cat# 705-545-147, RRID: AB_2336933 ), Alexa 10 Fluor® 488-conjugated donkey anti-rat (Cat# 712-545-150, RRID: AB_2340683 ), Alexa 10 Fluor® 488-conjugated donkey rabbit (Cat# 711-545-152, RRID: AB_2313584 ), Cy3-conjugated donkey anti-goat (Cat# 705-165-147, RRID: AB_2307351 ), Cy3-conjugated donkey anti-rabbit (Cat# 711-165-152, RRID: AB_2307443 ), Cy3-conjugated donkey anti-rat (Cat# 712-165-150, RRID: AB_2340666 ), Alexa 10 Fluor® 647-conjugated donkey anti-goat (Cat# 705-605-147, RRID: AB_2340437 ), Cy5-conjugated donkey anti-goat (Cat# 705-175-147, RRID: AB_2340415 ), Cy5-conjugated donkey anti-goat (Jackson ImmunoResearch Labs Cat# 705-175-147, RRID: AB_2340415 ) and Cy5-conjugated donkey anti-rat (Cat# 712-175-150, RRID: AB_2340671 ). .. Hoechst (1/1000; Sigma) was used for fluorescent nuclear counterstaining.

    Immunohistochemistry:

    Article Title: S1PR1 regulates the quiescence of lymphatic vessels by inhibiting laminar shear stress-dependent VEGF-C signaling.
    Article Snippet: Goat anti–human PROX1 (catalog AF2727), goat anti–mouse VEGRF3 (catalog AF743), goat anti–mouse NRP2 (catalog AF567), rat anti–mouse S1PR1 (catalog MAB7089), and goat anti–mouse LYVE-1 (catalog AF2125) were from R&D Systems. .. Secondary antibodies for IHC include Cy3-conjugated donkey anti-rabbit, Cy3-conjugated donkey anti-rat, Cy3-conjugated donkey anti-goat, Cy5-conjugated donkey anti-rat, Cy5-conjugated donkey anti-goat, FITC-conjugated donkey anti-chicken, Alexa 488–conjugated donkey anti-goat, and Biotin-conjugated donkey anti-goat antibodies, purchased from Jackson ImmunoResearch Laboratories (catalogs 711-165-152, 712-165-153, 705-165-147, 712-175-150, 705-175-147, 703-095-155, 705-545- 147, and 705-065-147, respectively). .. Alexa 488–conjugated donkey anti-rat antibody was purchased from Invitrogen (catalog A-21208).



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